TMIG Proteome Database of SH-SY5Y in XML
SH-SY5Y, human dopaminergic neuroblast
cell line
The neuroblastoma cell line SH-SY5Y (SK-N-SH-SY5Y), which was
subcloned from human neuroblastoma SK-N-SH, is a nearly diploid
dopaminergic neuronal cell line. When grown in 15% fetal calf serum in
Ham's F-12 medium as herein described, SH-SY5Y has neuroblast-like
properties in its undifferentiated state. Treatment with nerve growth
factor results in morphological and physiological differentiation not
unlike that elicited by nerve growth factor in sympathetic ganglia
primary cultures. Careful examination of catecholamine synthesizing
enzyme specific activities and catecholamine concentrations in nerve
growth factor-treated SH-SY5Y cells showed a small elevation of
tyrosine hydroxylase, no change in dopamine-beta-hydroxylase or in
dopamine or norepinephrine intracellular concentrations. Further,
treatment of SH-SY5Y cells with retinoic acid results in the cells
withdrawing from the cell cycle and extending neurites, in the same
time frame that tissue transglutaminase expression significantly
increases. The SH-SY5Y cell line is a convenient resource for
researches on neuronal cell biology in vitro.
The clickable 2-D gel protein map of
SH-SY5Y cell line. 
IPG strip used: Immobiline DryStrip (18 cm), pH 4-7; Code No.
17-1233-01; Lot No. 236318
This database is presented by Toda, T., Nakamura, M., Osawa, T.,
Hirota, M. and Morisawa H.