TMIG Proteome Database of TIG-3 for Cellular Aging Research

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TIG-3: normal human diploid fibroblast cell strain

The cell strain TIG-3, which was established in our institute, shows the finite life span up to 70-80 population doubling levels (PDLs). The cell strain has been widely used for researches on molecular mechanisms of replicative cell aging and immortalization. The preparation of our 2-D gel-based proteome database was intended for promoting proteomic researches on cellular aging. (Toda T, Kaji K, Kimura N. Electrophoresis 19(2), 344-348, 1998) (Toda T, Satoh M, Sugimoto M, Goto M, Furuichi Y, Kimura N. Mech Ageing Dev 100(2):133-143, 1998) (Toda T. Experimental Gerontology 35: 803-810, 2000 (PDF)) ( Toda T. Ann N Y Acad Sci 928:71-78, 2001)

The clickable 2-D gel image map:
The colored symbols indicate alteration in each protein spot during in-vitro aging.

IPG strip used: Immobiline DryStrip (18 cm), pH 4-7; Code No. 17-1233-01; Lot No. 236318
This database is presented by Toda, T., Kaji, K., and Kimura, N.

Download MALDI-TOF MS data (in mzXML files)

Download MS data viewer installer (MS_setup.exe for Windows only)

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• An alternative image map with identified protein names
  
  
• The raw image for spot matching by an image analyzer
  
  

• The list of spot protein data
  
  
• The morphplogical alteration of TIG-3 cells with aging.
  
  

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